Ma. Ragan et al., A NOVEL CLADE OF PROTISTAN PARASITES NEAR THE ANIMAL-FUNGAL DIVERGENCE, Proceedings of the National Academy of Sciences of the United Statesof America, 93(21), 1996, pp. 11907-11912
Sequences of nuclear-encoded small-subunit rRNA genes have been determ
ined for representatives of the enigmatic genera Dermocystidium, Ichth
yophonus, and Psorospermium, protistan parasites of fish and crustacea
ns. The small-subunit rRNA genes from these parasites and from the ''r
osette agent'' (also a parasite of fish) together form a novel, statis
tically supported clade. Phylogenetic analyses demonstrate this clade
to diverge near the animal-fungal dichotomy, although more precise res
olution is problematic In the most parsimonious and maximally likely p
hylogenetic frameworks inferred from the most stably aligned sequence
regions, the clade constitutes the most basal branch of the metazoa; b
ut within a limited range of model parameters, and in some analyses th
at incorporate less well-aligned sequence regions, an alternative topo
logy in which it diverges immediately before the animal-fungal dichoto
my was recovered. Mitochondrial cristae of Dermocystidium spp. are fla
t, whereas those of Ichthyophonus hoferi appear tubulovesiculate. Thes
e results extend our understanding of the types of organisms from whic
h metazoa and fungi may have evolved.