FUNCTIONAL ANTAGONISM BY A MONOCLONAL-ANTIBODY TO DIGOXIN IN A TEST SYSTEM OF CULTURED RAT-HEART MYOCYTES

Authors
WALLUKAT G SIMON HU MULLER WD WOLF I
Citation
G. Wallukat et al., FUNCTIONAL ANTAGONISM BY A MONOCLONAL-ANTIBODY TO DIGOXIN IN A TEST SYSTEM OF CULTURED RAT-HEART MYOCYTES, Molecular and cellular biochemistry, 161, 1996, pp. 117-120
Citations number
27
Categorie Soggetti
Biology,"Cell Biology
Journal title
Molecular and cellular biochemistry → ACNP
ISSN journal
03008177
Volume
161
Year of publication
1996
Pages
117 - 120
Database
ISI
SICI code
0300-8177(1996)161:<117:FABAMT>2.0.ZU;2-O
Abstract
A monoclonal antibody with a high affinity for digitoxin (K-A = 0.50 n M) and digoxin (K-A = 0.55 nM) was produced by somatic cell fusion. Th is antibody, designated 2A3(47), displayed little cross reactivity wit h other glycosides. In cultured rat heart myocytes, 2A3(47), antagoniz ed the positive chronotropic effect exerted by digitoxin but did not a lter that of ouabain. Our results suggest that this monoclonal antibod y may prove to be useful in treating digoxin and digitoxin intoxicatio n.