HIGH-THROUGHPUT PLASMID MINI PREPARATIONS FACILITATED BY MICRO-MIXING

We have developed a reliable high-throughput plasmid isolation system using a 96-well plate format. This system combines a novel glass bead micro-mixing method with modified alkaline lysis and Sephacryl S-500 D NA purification procedures. Mechanical forces generated by vortexing g lass beads inside each well of the 96-well plates ensure that the bact erial pellets are homogeneously resuspended, the cells are completely lyzed, and the resulting bacterial lysates are thoroughly mixed with t he potassium acetate solution, The vortexing speed and duration for gl ass bead mixing have been standardized to facilitate plasmid DNA yield s without significant adjustments.