INFLUENCE OF 6-HYDROXYDOPAMINE LESION OF THE DOPAMINERGIC NIGROSTRIATAL PATHWAY ON THE MUSCLE TONE AND ELECTROMYOGRAPHIC ACTIVITY MEASURED DURING PASSIVE MOVEMENTS
S. Wolfarth et al., INFLUENCE OF 6-HYDROXYDOPAMINE LESION OF THE DOPAMINERGIC NIGROSTRIATAL PATHWAY ON THE MUSCLE TONE AND ELECTROMYOGRAPHIC ACTIVITY MEASURED DURING PASSIVE MOVEMENTS, Neuroscience, 74(4), 1996, pp. 985-996
The aim of the present study was to find out whether a 6-hydroxydopami
ne-induced lesion of the substantia nigra in rats would evoke muscular
rigidity of the parkinsonian type. Simultaneous measurements of muscl
e resistance (mechanomyogram) of the hind foot to passive flexion and
extension at the ankle joint, as well as of the electromyographic acti
vity of the antagonistic muscles of the ankle joint-the gastrocnemius
and tibialis anterior-in rats were carried out one, two and four weeks
after bilateral injections of 6-hydroxydopamine (6.5 mu g/mu l) into
the substantia nigra. After immunohistochemical staining of brain sect
ions for tyrosine hydroxylase, the rats were divided into two groups i
n which, on average, either 70% (63-80%) or 89% (81-96%) of nigral cel
ls degenerated. Larger lesions increased the resistance (mechanomyogra
m) of the rat's hind leg to passive movements two weeks after 6-hydrox
ydopamine injection, whereas smaller lesions did not. Muscle rigidity
was accompanied by an increase in the movement-induced reflex electrom
yographic activity in both muscles, mainly in long-latency components
which are most probably influenced by supraspinal mechanisms. However,
in spite of relatively large lesions of nigral dopamine cells, alread
y four weeks after the lesion, muscle rigidity and the respective elec
tromyographic activity diminished dramatically, which seems to result
from very effective compensatory mechanisms operating in young lesione
d rats. The results suggest that the muscle rigidity induced by the 6-
hydroxydopamine nigral lesion seems to be a good model of parkinsonian
rigidity. Copyright (C) 1996 IBRO. Published by Elsevier Science Ltd.