Z. Mallat et al., EVIDENCE OF APOPTOSIS IN ARRHYTHMOGENIC RIGHT-VENTRICULAR DYSPLASIA, The New England journal of medicine, 335(16), 1996, pp. 1190-1196
Background Arrhythmogenic right ventricular dysplasia, a disorder that
may lead to severe ventricular arrhythmias and sudden death, is chara
cterized by the progressive replacement of myocardial cells by fat and
fibrous tissue. We examined whether the loss of myocardial cells in t
his disease could result from cell death by apoptosis (programmed cell
death). Methods Specimens obtained at autopsy from the right ventricu
lar myocardium of eight patients with arrhythmogenic right Ventricular
dysplasia and four age-matched normal subjects were analyzed. To iden
tify individual cells undergoing apoptosis, we performed in situ end-l
abeling of fragmented DNA on paraffin sections using biotinylated deox
yuridine triphosphate and the enzyme terminal deoxynucleotidyl transfe
rase, We also examined the level of expression of CPP-32, a cysteine p
rotease required for apoptotic cell death in mammalian cells, using im
munohistochemical techniques. Results Apoptosis was detected in the ri
ght ventricular myocardium of six of the eight patients with arrhythmo
genic right ventricular dysplasia and was absent in the controls, High
levels of expression of CPP-32 were associated with positive in situ
end-labeling of fragmented DNA. Conclusions These results indicate tha
t apoptotic myocardial cell death occurs in arrhythmogenic right ventr
icular dysplasia and may contribute to the loss of myocardial cells in
this disorder. (C) 1996, Massachusetts Medical Society.