USE OF AN ALKALINE PHOSPHATASE-CONJUGATED OLIGONUCLEOTIDE PROBE FOR THE GENE ENCODING THE BUNDLE-FORMING PILUS OF ENTEROPATHOGENIC ESCHERICHIA-COLI

Authors
NAGAYAMA K BI Z OGUCHI T TAKARADA Y SHIBATA S HONDA T
Citation
K. Nagayama et al., USE OF AN ALKALINE PHOSPHATASE-CONJUGATED OLIGONUCLEOTIDE PROBE FOR THE GENE ENCODING THE BUNDLE-FORMING PILUS OF ENTEROPATHOGENIC ESCHERICHIA-COLI, Journal of clinical microbiology, 34(11), 1996, pp. 2819-2821
Citations number
20
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
34
Issue
11
Year of publication
1996
Pages
2819 - 2821
Database
ISI
SICI code
0095-1137(1996)34:11<2819:UOAAPO>2.0.ZU;2-F
Abstract
An alkaline phosphatase-conjugated 29-base oligonucleotide probe was d eveloped to detect the gene encoding the bundle-forming pilus of enter opathogenic Escherichia coli. The sensitivity and specificity of the p robe versus the results of localized adherence in the HEp-2 cell assay and fluorescent actin staining assay positivity were 95.7 and 100%, r espectively.