Es. Didier et al., A MICROSPORIDIAN ISOLATED FROM AN AIDS PATIENT CORRESPONDS TO ENCEPHALITOZOON-CUNICULI-III, ORIGINALLY ISOLATED FROM DOMESTIC DOGS, Journal of clinical microbiology, 34(11), 1996, pp. 2835-2837
The ribosomal DNA internal transcribed spacer (ITS) region of a recent
ly cultured human Encephalitozoon cuniculi isolate was analyzed by gen
e amplification and DNA sequencing. Restriction endonuclease digestion
(FokI) and double-stranded DNA heteroduplex mobility shift analysis w
ere performed to determine their utility for strain differentiation. T
he human E. cuniculi isolate was identical to E. cuniculi III, which h
ad been isolated only from domestic dogs until now. The patient provid
ing the isolate owned a pet dog, but no microsporidia were detected in
the pet's urine.