Jh. Jorgensen et al., COMPARISON OF INOCULATION METHODS FOR TESTING ENTEROCOCCI BY USING VANCOMYCIN SCREENING AGAR, Journal of clinical microbiology, 34(11), 1996, pp. 2841-2842
One hundred four recent clinical isolates of Enterococcus species mere
screened for vancomycin resistance by using inocula of 10(5) or 10(6)
CFU dispensed by pipet and by use of a cotton swab dipped in a 0.5 Mc
Farland standard organism suspension applied to the surface of brain h
eart infusion agar containing 6 mu g of vancomycin per ml. The three i
noculation methods were equivalent in the detection of nonsusceptible
isolates. The use of swab inoculation mas convenient and less costly t
han the use of micropipets.