PHOTOSELECTION OF LUMINESCENT MOLECULES IN ANISOTROPIC MEDIA IN THE CASE OF 2-PHOTON EXCITATION .2. EXPERIMENTAL STUDIES OF HOECHST-33342 IN STRETCHED POLY(VINYL ALCOHOL) FILMS
A. Kawski et al., PHOTOSELECTION OF LUMINESCENT MOLECULES IN ANISOTROPIC MEDIA IN THE CASE OF 2-PHOTON EXCITATION .2. EXPERIMENTAL STUDIES OF HOECHST-33342 IN STRETCHED POLY(VINYL ALCOHOL) FILMS, Zeitschrift fur Naturforschung. A, A journal of physical sciences, 51(9), 1996, pp. 1037-1041
It was found by investigating dichroism and emission anisotropy in the
case of one- and two-photon excitation of Hoechst 33342 [bis-benzimid
e,2,5'-bi- 1H-benzimidazole, 2'-(4-ethoxyphenyl)-5-5(4-methyl-1-pipera
zinyl)] in stretched poly(vinyl alcohol) (PVA) films, that the absorpt
ion and fluorescence transition moments lie along the long molecular a
xis of the molecule studied. The slight deviation of the transition mo
ment direction in fluorescence (about 8 degrees) from that in absorpti
on can be due to the incomplete linearity of the Hoechst molecule.