This paper is about how sciences relate to the phenomenal world. It fo
cusses on the construction of the empirical machineries involved in th
e creation of knowledge. The author adopts an ethnographic perspective
and contrasts two approachs: high energy physics and molecular biolog
y. In the first one the work of dealing with the referent is left to a
n interposed machine. Particle physics operates in a world separated f
rom the environment, reflexivity is turned into a principle of knowled
ge. In molecular biology, on the other hand, the epistemic culture is
oriented toward positive knowledge built from the manipulation of obje
ts in an analogous regime that continuously turns away from sign profe
sses.