S. Koster et al., INFLUENCE OF A CO2-PNEUMOPERITONEUM ON IN TRAPERITONEAL TUMOR-GROWTH IN AN ANIMAL-MODEL, Geburtshilfe und Frauenheilkunde, 56(9), 1996, pp. 458-461
Clinical observations during recent years describe the increased occur
rence of rapid intraperitoneal metastases and an impairment of prognos
is if a malignant tumour was operated laparoscopically, It is not know
n whether the observed Induction of intraabdominal metastases after la
paroscopic surgery in the result of an inadequate surcigal procedure w
ith spillage of tumour cells into the abdominal cavity or whether pneu
moperitoneum-induced changes of the peritoneal milieu promote tumour g
rowth, An animal model was established to examine the effect of a CO2-
pneumoperitoneum on the intraperitoneal growth of tumour cells, Six we
ek old nude mice were divided into 4 groups of 15 animals each, A CO2-
pneumoperitoneum was induced by a micro-hysteroflator with an intraper
itoneal pressure of 6 mmHg for 90 minutes in groups 2 and 4. Two diffe
rent tumour cell lines, suspended in 2 mi of Ringer's solution for hom
ogeneous spread of the cells, were injected i. p. The control groups 1
and received the tumour cells without pneumoperitoneum. The induction
of a CO2-pneumoperitoneum resulted in an increasing seeding rate of t
umour cells and provoked a significant increase in the number and size
of intraabdominal metastases. Laparoscopic surgery of malignancies is
therefore contraindicated.