Ll. Dezwart et al., DISPOSITION IN RAT OF [2-H-3]-TRANS-4-HYDROXY-2,3-NONENAL, A PRODUCT OF LIPID-PEROXIDATION, Xenobiotica, 26(10), 1996, pp. 1087-1100
1. 4-Hydroxy-2,3-nonenal (HNE) is an end product of lipid peroxidation
(LPO) and a well known cytotoxic aldehyde that exhibits a variety of
biological effects. In this study the in vivo disposition and covalent
binding of i.p. administered [2-H-3]HNE was examined in the rat. 2. I
t was found that several metabolites of [2-H-3]HNE are excreted in uri
ne among which at least four mercapturic acids. 1,4-Dihydroxynonane me
rcapturic acid (DHN-MA) appeared to be the most abundant mercapturic a
cid excreted in urine (3.5 % of the dose) and the excretion of the oth
er three mercapturic acids amounted to 2 % of the dose. 3. Within 48 h
following i.p. administration of 5 or 25 mu mol/kg bodyweight [2-H-3]
HNE (specific activity 4 mu Ci/mu mol) about 25 % of the radioactivity
was excreted in urine, whereas 18 % of the radioactivity appeared in
the faeces. 4. After 48 h, 7 % of the radioactivity was still present
in the liver and 0.2 % in other organs, but this radioactivity appeare
d not to be covalently bound to cellular macromolecules. It was found
that only 0.13 % of the radioactivity was covalently bound in the live
r and even less in the other organs.