PLANT GROWTH-PROMOTING RHIZOBACTERIA ALTER ROOTING PATTERNS AND ARBUSCULAR MYCORRHIZAL FUNGI COLONIZATION OF FIELD-GROWN SPRING WHEAT

The impact of plant growth-promoting rhizobacteria (PGPR) inoculants o n the growth, yield and interactions of spring wheat with arbuscular m ycorrhizal fungi (AMF) was assessed in field studies. The pseudomonad inoculants P. cepacia R55, R85, P. aeruginosa R80, P. fluorescens R92 and P. putida R104, which enhance growth and yield of winter wheat, we re applied at a rate of ca. 10(7)-10(8) cfu seed(-1) and plots establi shed on pea stubble or summer fallow at two different sites in Saskatc hewan. Plant shoot and root biomass, yield and AMF colonization were d etermined at four intervals. Plant growth responses were variable and dependent on the inoculant strain, harvest date and growth parameter e valuated. Significant increases or decreases were measured at differen t intervals but these were usually transient and final seed yield was not significantly affected. Harvest index was consistently increased b y all pseudomonad inoculants; responses to strain R55 and R104 were si gnificant. Root biomass to 60 cm depth was not significantly affected by inoculants except strain R104, which significantly reduced root dry weight. However, root distribution, root length and AMF colonization of roots within the soil profile to 60 cm were significantly altered b y inoculants. Most of these responses were reductions in the assessed parameter and occurred at depths below 15 cm; however, strains R85 and R92 significantly increased root dry weight in the 0- to 15-cm zone. These results indicate that some PGPR inoculants may adversely affect mutualistic associations between plants and indigenous soil microorgan isms, and suggest a possible reason as to why spring wheat growth was not consistently enhanced by these pseudomonad PGPR.