HIGH-PERFORMANCE-LIQUID-CHROMATOGRAPHY - ATMOSPHERIC-PRESSURE NEGATIVE CHEMICAL-IONIZATION - MASS-SPECTROMETRY OF 2,4-DINITROPHENYL DERIVATIVES OF AMINES

High-performance liquid chromatography (HPLC) coupled to mass spectrom etry (MS) is used to detect 2,4-dinitrophenyl (DNP) derivatives of ami nes by means of negative chemical ionisation at atmospheric pressure. The high sensitivity and good comparability of UV- and MS-detection of DNP-derivatives of amines is shown by several examples. The high sele ctivity of the derivatisation and the detection method (UV and MS) is used for the analysis of unknown amines in aqueous phases after hydrol ytic degradation of polyamide-amine- or polyamine-epoxide-adducts as w ell as for the characterisation of commercial products.