EFFECTS OF THE AMINO-ACID GLUTAMINE ON FREQUENCY OF CHROMOSOMAL-ABERRATIONS INDUCED BY GAMMA-RADIATION IN WISTAR RATS

The radiotherapy treatment of human cancer is often limited by the sid e effects and complications induced in normal surrounding tissues. The use of therapeutic strategies that could protect normal tissues while permitting the death of malignant neoplasm would be advantageous. Som e studies have suggested that the amino acid glutamine (GLN) can serve as a conditionally essential nutrient in patients in a catabolic cond ition. The objective of this study was to evaluate the possible radiop rotection of GLN on the frequency of chromosomal aberrations, number o f metaphases with chromosomal aberrations and mitotic index in bone ma rrow cells of Rattus norvegicus. In this in vivo test system, GLN was administered by gavage at concentrations of 300 and 600 mg/kg body wei ght, in acute treatments, 30 min or 24 h before exposure to 3 Gy of wh ole-body gamma radiation. The results obtained in these experiments sh owed that GLN did not alter significantly the frequency of chromosome aberrations induced by gamma radiation under the experimental conditio ns used in the present study.