CLONING AND SEQUENCING OF A CDNA-ENCODING BOVINE INTERCELLULAR-ADHESION-MOLECULE-3 (ICAM-3)

A cDNA encoding a putative bovine intercellular adhesion molecule (ICA M)-3, a ligand of the leukocyte integrin LFA-1 (CD11a/CD18), was seque nced and compared with human ICAM sequences. The 1635-bp bovine sequen ce codes for a protein of 544 amino acids (aa). This putative bovine I CAM-3 has five immunoglobulin (Ig)-like domains similar to human ICAM- 1 and ICAM-3, and belongs to the Ig gene superfamily. The overall iden tities of the deduced aa sequence with those of human ICAM-3 and ICAM- 1 are 61% and 58%, respectively. The predicted number and positions of Cys residues are all conserved between the bovine and human ICAM 3 aa sequences.