R. Ohi et al., CONSTRUCTION OF VECTORS AND A GENOMIC LIBRARY FOR USE WITH HIS3-DEFICIENT STRAINS OF SCHIZOSACCHAROMYCES-POMBE, Gene, 174(2), 1996, pp. 315-318
The construction of vectors for use in Schizosaccharomyces pombe using
the his3(+) gene as a selectable marker is described. In addition, we
report the construction of a genomic library in a his3(+)-containing
shuttle vector to facilitate the cloning of genes by complementation o
f mutant function in strains defective for His3 activity.