12 AMPLIFIED AND EXPRESSED GENES LOCALIZED IN A SINGLE-DOMAIN IN GLIOMA

Gene amplification has been associated both with tumor stage and progr ession in human gliomas. Several distinct amplified loci have been ide ntified by comparative genomic hybridization and Southern blot analysi s. It has been increasingly recognized that amplified domains comprise multiple genes. Here, we demonstrate amplification of up to 12 differ ent genes from an amplified domain at 12q13-15 that has been found in approximately 15% of astrocytomas and glioblastomas. The amplified gen es were GLI, WNT1, MDM2, SAS, CDK4, OS-4, GAS16, GAS27, GAS41, GAS56, GAS 64 and GAS89. In one glioblastoma all 12 amplified genes were also found to be expressed. These results strongly warrant the search for as yet unidentified genes in regions previously reported to be amplifi ed.