J. Weberarden et al., INVERSE PCR AMPLIFICATION OF LOW-ABUNDANCY MESSAGE OF GAMMA-DELTA T-CELL RECEPTOR GENES, Journal of immunological methods, 197(1-2), 1996, pp. 187-192
The technique of inverse PCR permits the rapid amplification and ident
ification of unknown DNA segments adjacent to well characterized core
regions. In the field of immunology anchored PCR and inverse PCR are u
seful methods for examining junctional diversity and unknown variable
gene segments of rearranged T cell receptor genes. We have applied an
improved inverse PCR protocol to study the repertoire of gamma delta T
cell receptor genes in the developing thymus of the mouse.