A SET OF GENETIC-MARKERS FOR THE CHROMOSOMES OF THE IMPERFECT YEAST ARXULA-ADENINIVORANS

The nuclear genome of the anamorphic yeast Arxula adeninivorans was an alysed by benomyl-induced haploidization of parasexual hybrids marked with 32 auxotrophic mutations and pulsed field gel electrophoresis fol lowed by DNA hybridization. Twenty-seven genes have been arranged into four linkage groups by haploidization, 15 genes belong to group 1, si x to group 2, and three each to groups 3 and 4. Five genes could be lo calized by DNA hybridization on three out of four separated chromosome s. The gene LYS2 of the largest linkage group 1 and the 25S rDNA were identified on the largest chromosome, the GAA and the TEF1 gene on chr omosome 2, and the ILV1 gene of linkage group 4 on the smallest chromo some.