A RAPID ANTIBODY-BASED TEST FOR SEC-2, A MARKER FOR THE SHORT ARM OF CHROMOSOME-2 OF RYE (2RS)

A simple monoclonal antibody-based screening test has been developed f or the presence of translocations of the short arm of chromosome 2 of rye (2RS) with wheat chromosome 2B. 2RS encodes a set of about three p olypeptides known as M(r) 75 000 gamma-secalins. Use of the antibody t est for these secalins enabled screening of several hundred seeds per day. The antibody could readily distinguish 2BL-2RS translocations and 2R substitutions from 1BL-1RS translocations or nontranslocation whea ts. Use of the antibody in analysis of segregating progeny for Sec-2 i n several wheat backgrounds was successful. Results with a selection o f the seed population were checked using protein gel electrophoresis, with 100% correct confirmation.