PROTEIN-KINASE-C INACTIVATION BY FENTONS-REACTION AT DISCRETE CU-SITES(+ BINDING)

Authors
TRAVERSO N RICCIARELLI R DOMENICOTTI C MENINI S PRONZATO MA COTTALASSO D MARINARI UM
Citation
N. Traverso et al., PROTEIN-KINASE-C INACTIVATION BY FENTONS-REACTION AT DISCRETE CU-SITES(+ BINDING), Biochemistry and molecular biology international, 40(2), 1996, pp. 285-293
Citations number
29
Categorie Soggetti
Biology
Journal title
Biochemistry and molecular biology international → ACNP
ISSN journal
10399712
Volume
40
Issue
2
Year of publication
1996
Pages
285 - 293
Database
ISI
SICI code
1039-9712(1996)40:2<285:PIBFAD>2.0.ZU;2-7
Abstract
The consequence of direct exposure to HO. radical (chemically generate d by Fenton's reaction) of partially purified rat liver PKC has been e valuated in this work. PKC inhibited Fenton-dependent HO. generation, probably due to the binding of copper ions to the enzyme. PKC activity was inhibited by H2O2. Copper ions were able to increase the H2O2-med iated damage to the enzyme, but only beyond a concentration threshold. The possible interactions between PKC and Fenton's reagents, in parti cular copper ions, is discussed.