EFFECTS OF STRESS-RELATED SIGNAL MOLECULES ON CELLS ASSOCIATED WITH MUSCLE-TISSUE

OBJECTIVE: To elucidate the physiologic background that makes muscle h ypertrophy, especially that due to strenuous exercise, often parallel to stress sensitivity and signs of acute phase immune response. STUDY DESIGN: We used an animal model: lines of mice with hypertrophied (H) and normally developed (N) hind leg muscles, six in each case. Functio nal and receptor tests on cells from digested muscle tissue were made and analyzed by microplate cytofluorimetry and flow cytometry. RESULTS : Higher percentages of cells with a phagocyte marker (> 2-fold) and w ith opioid (about 1.3-fold) receptors were found, whereas the portion of glucocorticoid receptor-bearing cells tended to differ only among H and N. Naloxone, an opioid receptor antagonist, failed only in H to e xert a suppressive effect on dihydrorhodamine 123 oxidation. CONCLUSIO N: These results and differences in responses of lipid trafficking and proteolytic activities to cortisol, naloxone and adrenergic receptor agonists suggest that not only the cell population associated with mus cle tissue but also receptor-mediated responses that are known to be r elated to stress coping are different between H and N.