COMPARISON OF LIQUID AND SUPERCRITICAL-FLUID CHROMATOGRAPHY USING NAPHTHYLETHYLCARBAMOYLATED-BETA-CYCLODEXTRIN CHIRAL STATIONARY PHASES

A comparison of liquid (LC) and sub- or supercritical fluid chromatogr aphy (SFC) was performed using naphthylethylcarbamoylated-beta-cyclode xtrin chiral stationary phases (NEC-CD CSPs). Compounds resolved in th e normal-phase, reversed-phase and polar organic modes on the NEC-CD C SPs in LC were also separated on the same columns in SFC with a carbon dioxide-alcohol eluent. Advantages of SFC for chiral separations on t hese CSPs included simple eluents, rapid optimization of selectivity a nd improved resolution compared to LC results for the same columns. Th e effect of alcohol modifier was also investigated and the results pro vided insights into the chiral recognition mechanisms in SFC. The vers atility of the NEC-CD CSPs in SFC was demonstrated by performing separ ations representative of the three distinct mobile phase modes in LC i n a single analysis in SFC.