CHROMATOGRAPHIC AND ELECTROPHORETIC METHODS FOR ANALYSIS OF SUPEROXIDE DISMUTASES

A brief overview of the family of superoxide dismutase (SOD) enzymes a nd their biomedical significance is presented. Methodology for the pur ification and electrophoretic analysis of superoxide dismutases is rev iewed and discussed, with emphasis on the specific problems raised by the separation of individual superoxide dismutase isoenzymes. Purifica tion methods and their performance, as reported in the literature, are summarised in table form. Generally used methods for measuring SOD ac tivity in vitro and SOD visualisation after electrophoresis are outlin ed, particularly those relevant to the monitoring of progress of SOD p urification.