EVIDENCE FROM KINETIC ISOTOPE STUDIES FOR AN ENOLATE INTERMEDIATE IN THE MECHANISM OF TYPE-II DEHYDROQUINASES

Authors
HARRIS JM GONZALEZBELLO C KLEANTHOUS C HAWKINS AR COGGINS JR ABELL C
Citation
Jm. Harris et al., EVIDENCE FROM KINETIC ISOTOPE STUDIES FOR AN ENOLATE INTERMEDIATE IN THE MECHANISM OF TYPE-II DEHYDROQUINASES, Biochemical journal, 319, 1996, pp. 333-336
Citations number
20
Categorie Soggetti
Biology
Journal title
Biochemical journal → ACNP
ISSN journal
02646021
Volume
319
Year of publication
1996
Part
2
Pages
333 - 336
Database
ISI
SICI code
0264-6021(1996)319:<333:EFKISF>2.0.ZU;2-Y
Abstract
Substrate isotope studies, solvent isotope studies, proton inventories and studies of V-max and K-m as a function of pH suggest an E(1)CB (V . E. Anderson (1991) in Enzyme Mechanisms for Isotope Effects (Cook, P . F., ed.), pp. 389-417, CRC Press, Boca Raton, FL) mechanism via an e nolate intermediate for type II dehydroquinases involved in biosynthet ic or catabolic pathways.