INCREASED SELECTIVE UPTAKE IN-VIVO AND IN-VITRO OF OXIDIZED CHOLESTERYL ESTERS FROM HIGH-DENSITY-LIPOPROTEIN BY RAT-LIVER PARENCHYMAL-CELLS

Oxidation of low-density lipoprotein (LDL) leads initially to the form ation of LDL-associated cholesteryl ester hydroperoxides (CEOOH). LDL- associated CEOOH can be transferred to high-density lipoprotein (HDL), and HDL-associated CEOOH are rapidly reduced to the corresponding hyd roxides (CEOH) by an intrinsic peroxidase-like activity. We have now p erformed in vivo experiments to quantify the clearance rates and to id entify the uptake sites of HDL-associated [H-3]Ch18:2-OH in rats. Upon injection into rats, HDL-associated [H-3]Ch18:2-OH is removed more ra pidly from the circulation than HDL-associated [H-3]Ch18:2. Two minute s after administration of [H-3]Ch18:2-OH-HDL, 19.6 +/- 2.6 % (S.E.M.; n = 4) of the label was taken up by the liver as compared with 2.4 +/- 0.25 % (S.E.M.; n = 4) for [H-3]Ch18:2-HDL. Organ distribution studie s indicated that only the liver and adrenals exhibited preferential up take of [H-3]Ch18:2-OH as compared with [H-3]Ch18:2, with the liver as the major site of uptake. A cell-separation procedure, employed 10 mi n after injection of [H-3]Ch18:2-OH-HDL or [H-3]Ch18:2-HDL, demonstrat ed that within the liver only parenchymal cells take up HDL-CE by the selective uptake pathway, Selective uptake by parenchymal cells of [H- 3]Ch18:2-OH was 3-fold higher than that of [H-3]Ch18:2, while Kupffer and endothelial cell uptake of the lipid tracers reflected HDL holopar ticle uptake (as analysed with iodinated versus cholesteryl ester-labe lled HDL), The efficient uptake of [H-3]Ch18:2-OH by parenchymal cells was coupled to a 3-fold increase in rate of radioactive bile acid sec retion from [H-3]Ch18:2-OH-HDL as compared with [H-3]Ch18:2-HDL. In vi tro studies with freshly isolated parenchymal cells showed that the as sociation of [H-3]Ch18:2-OH-HDL at 37 degrees C exceeded [H-3]Ch18:2-H DL uptake almost 4-fold. Our results indicate that HDL-associated CEOH are efficiently and selectively removed from the blood circulation by the liver in vivo. The selective liver uptake is specifically exerted by parenchymal cells and coupled to a rapid biliary secretion pathway . The liver uptake and biliary secretion route may allow HDL to functi on as an efficient protection system against potentially atherogenic C EOOH.