I. Dalmau et al., REDUCTION OF THE MICROGLIAL CELL NUMBER IN RAT PRIMARY GLIAL-CELL CULTURES BY EXOGENOUS ADDITION OF DIBUTYRYL CYCLIC ADENOSINE-MONOPHOSPHATE, Journal of neuroimmunology, 70(2), 1996, pp. 123-129
The present work examined the effects induced by dibutyryl cyclic aden
osine monophosphate (dB-cAMP) on microglial cells in primary glial cel
l cultures from newborn rats. Microglial cells were identified by OX42
immunohistochemistry and nucleoside diphosphatase histochemistry. Dou
ble staining for astrocytes was carried out by combination with glial
fibrillary acidic protein immunolabeling. Addition of 0.25 mM dB-cAMP
to the cultures decreased the microglial cell number about sixfold. Th
e findings suggest that the effect of dB-cAMP on the microglial cells
might be either a direct action of dB-cAMP on the microglial cells or
an indirect effect mediated by the astroglial cells.