A SIMPLIFIED COCULTURE SYSTEM WITH LUTEINIZED GRANULOSA-CELLS IMPROVES EMBRYO QUALITY AND IMPLANTATION RATES - A CONTROLLED-STUDY

Objective: To investigate the use of a simplified short-term coculture system with luteinized granulosa cells (GCs) in patients with failed IVF-ET. Design: Controlled clinical study. Setting: IVF unit, Departme nt of Obstetrics and Gynecology, Carmel Medical Center. Patient(s): Pa tients with poor embryo quality in their previous IVF-ET cycles.Interv ention(s): Embryos from 40 patients, in which > 50% of the embryos wer e classified as poor quality in their previous IVF attempts, were grow n on autologue GC culture system for a short period (24-48 hours) befo re being replaced in the uterine cavity. Main Outcome Measure(s): Embr yo quality. Result(s): Significant decrease in poor quality embryos an d increase in the proportion of good quality embryos were observed usi ng a coculture system with autologue human GCs. Pregnancy rates in thi s groups of patients reached our standard IVF results during the same period. Conclusion(s): This study describes: a simplified short-term c oculture system with human autologue GCs. Poor quality embryos may be rescued to cleave regularly.