DETERMINATION OF OPTIMUM CONDITIONS FOR I MMOBILIZATION OF ALPHA-AMYLASE ON CORN GRITS

Conditions for immobilization of alpha-amilase on corn grits were exam ined in relation to the retention of the enzymatic activity. The oxidi zation stage is the most influential in the inmobilization process, th e retention activity increase with the sodium periodate concentration. However, when concentration was superior to 2000 mg periodate/g corn, the corn was broken. The operational stability of the immobilizated e nzyme indicated that the best bioparticles was obtained when the conce ntration used was 1400 mg periodate/g corn.