ESTIMATIONS OF AVERAGE PARTICLE SIZES AND SIZE DISTRIBUTIONS OF COMMERCIALLY AVAILABLE HUMAN-INSULIN AQUEOUS SUSPENSIONS USING LASER-LIGHT DIFFRACTION SPECTROSCOPY

Citation
H. Komatsu et al., ESTIMATIONS OF AVERAGE PARTICLE SIZES AND SIZE DISTRIBUTIONS OF COMMERCIALLY AVAILABLE HUMAN-INSULIN AQUEOUS SUSPENSIONS USING LASER-LIGHT DIFFRACTION SPECTROSCOPY, Chemical and Pharmaceutical Bulletin, 44(10), 1996, pp. 1966-1969
Citations number
15
Categorie Soggetti
Pharmacology & Pharmacy",Chemistry
ISSN journal
00092363
Volume
44
Issue
10
Year of publication
1996
Pages
1966 - 1969
Database
ISI
SICI code
0009-2363(1996)44:10<1966:EOAPSA>2.0.ZU;2-2
Abstract
The average diameter and size distribution of particles in commerciall y available isophane-insulin and zinc-insulin aqueous suspensions (11 products) were examined using laser-light diffraction spectroscopy (LL DS), Wide variation was observed between products in volume-weighted a nd number-weighted average diameters, The particles in the insulin aqu eous suspensions were found to be mono-dispersed within relatively nar row size distributions, Sonication for a short time led to a downshift in the apparent average diameter, which was most remarkable with isop hane-insulin aqueous suspensions, This could be ascribed to dispersion of aggregated needle-like crystals and/or crystal breakage, In the ca se of biphasic isophane-insulin aqueous suspensions, the average size as well as the size distribution shifted toward the smaller end of the scale with decrease in the isophane content.