ESTIMATIONS OF AVERAGE PARTICLE SIZES AND SIZE DISTRIBUTIONS OF COMMERCIALLY AVAILABLE HUMAN-INSULIN AQUEOUS SUSPENSIONS USING LASER-LIGHT DIFFRACTION SPECTROSCOPY
H. Komatsu et al., ESTIMATIONS OF AVERAGE PARTICLE SIZES AND SIZE DISTRIBUTIONS OF COMMERCIALLY AVAILABLE HUMAN-INSULIN AQUEOUS SUSPENSIONS USING LASER-LIGHT DIFFRACTION SPECTROSCOPY, Chemical and Pharmaceutical Bulletin, 44(10), 1996, pp. 1966-1969
The average diameter and size distribution of particles in commerciall
y available isophane-insulin and zinc-insulin aqueous suspensions (11
products) were examined using laser-light diffraction spectroscopy (LL
DS), Wide variation was observed between products in volume-weighted a
nd number-weighted average diameters, The particles in the insulin aqu
eous suspensions were found to be mono-dispersed within relatively nar
row size distributions, Sonication for a short time led to a downshift
in the apparent average diameter, which was most remarkable with isop
hane-insulin aqueous suspensions, This could be ascribed to dispersion
of aggregated needle-like crystals and/or crystal breakage, In the ca
se of biphasic isophane-insulin aqueous suspensions, the average size
as well as the size distribution shifted toward the smaller end of the
scale with decrease in the isophane content.