Hh. Koster et al., EFFECT OF INCREASING DEGRADABLE INTAKE PROTEIN ON INTAKE AND DIGESTION OF LOW-QUALITY, TALLGRASS-PRAIRIE FORAGE BY BEEF-COWS, Journal of animal science, 74(10), 1996, pp. 2473-2481
Five ruminally and duodenally fistulated Angus x Hereford cows were us
ed in a 5 x 5 Latin square to monitor intake, ruminal fermentation res
ponses, and site and extent of digestion associated with providing inc
reasing amounts of supplemental degradable intake protein (DIP). Cows
had ad libitum access to low-quality, tallgrass-prairie forage (1.9% C
P, 77% NDF) that was fed twice daily. The supplemental DIP (sodium cas
einate; 90% CP) was infused intraruminally at 0630 and 1830 immediatel
y before feeding forage. Levels of DIP were 0, 180, 360, 540, and 720
g/d. Each period consisted of 14 d of adaptation and 6 d of sampling.
Forage OM intake increased quadratically (P < .01) with increasing sup
plemental DIP, reaching a peak at the 540 g/d level. True ruminal OM a
nd NDF digestion increased with the addition of 180 g/d supplemental D
IP, but exhibited only moderate and somewhat variable responses when g
reater amounts of supplemental DIP were infused (cubic, P < .03). Micr
obial N flow and efficiency increased linearly (P < .01) with increasi
ng supplemental DIP. However, a quadratic effect (P < .01) was observe
d for total duodenal N flow, which was maximized at 540 g/d supplement
al DIP. A linear (P = .02) treatment effect was observed for ruminal f
luid dilution rate. Total ruminal VFA and ammonia concentrations incre
ased (P < .01) in response to DIP supplementation. In conclusion, incr
easing supplemental DIP generally improved forage utilization; intake
of digestible OM was maximized when it contained approximately 11% DIP
.