GENE LOCALIZATION AND SYNTENIC MAPPING BY FISH IN THE DOG

To begin development of a canine gene map and to define syntenic regio ns between the canine and human genomes, the technique of fluorescence in situ hybridization (FISH) was adapted to localize specific DNA seq uences in canine metaphases. Genomic clones of canine origin as well a s human yeast artificial chromosome clones were used to map canine loc i. The immunoglobulin heavy-chain region was localized to canine chrom osome 4qtel; the canine major histocompatibility locus, DLA, was found to be on chromosome 12qtel. Of particular interest, the canine X chro mosome, which morphologically is highly similar to the human X, also s howed syntenic localization of the clotting factor VIII (F8C) and fact or IX (F9) genes to Xq28 and Xq26.3-->q27.1, respectively.