SIMULTANEOUS OBTENTION OF AN INTENSE G-BANDING AND CHROMOSOME PAINTING

Citation
F. Richard et B. Dutrillaux, SIMULTANEOUS OBTENTION OF AN INTENSE G-BANDING AND CHROMOSOME PAINTING, Cytogenetics and cell genetics, 74(1-2), 1996, pp. 124-126
Citations number
17
Categorie Soggetti
Cell Biology","Genetics & Heredity
ISSN journal
03010171
Volume
74
Issue
1-2
Year of publication
1996
Pages
124 - 126
Database
ISI
SICI code
0301-0171(1996)74:1-2<124:SOOAIG>2.0.ZU;2-F
Abstract
A method for simultaneously obtaining brilliant G-banding and chromoso me painting is described, It is based on a modification of methods ena bling the detection of incorporated BrdU by anti-BrdU antibodies conju gated to a fluorochrome, It induces a banding of both painted and non- painted chromosomes. The method was adapted for in situ hybridizations on human and non human (ZOO-FISH) chromosomes.