SEQUENCE-ANALYSIS AND GENETIC-MAPPING OF PORCINE CHROMOSOME-11 CENTROMERIC S0048 MARKER

We report the existence of a new family of swine centromeric satellite DNA composed of a 51-bp repeat unit, most specifically found on pig c hromosome 11 centromere and with less specificity at the centromeric r egion of other meta- and submetacentric chromosomes. This satellite DN A family, which has no homologies with the Mc1 and Ac2 families publis hed previously, was named Mc2. We designed a specific primer set for P CR amplification of this centromeric satellite DNA. Specificity of amp lification was checked by using a porcine somatic cell hybrid panel an d by FISH. Furthermore, the development of a PCR-RFLP marker of Mc2 re petition allowed its genetic mapping on the PiGMaP reference families panel. The centromere of chromosome 11 was thus integrated to the gene tic map previously published.