MOLECULAR-CLONING OF UBE2G, ENCODING A HUMAN SKELETAL MUSCLE-SPECIFICUBIQUITIN-CONJUGATING ENZYME HOMOLOGOUS TO UBC7 OF C-ELEGANS

Authors
WATANABE TK KAWAI A FUJIWARA T MAEKAWA H HIRAI Y NAKAMURA Y TAKAHASHI E
Citation
Tk. Watanabe et al., MOLECULAR-CLONING OF UBE2G, ENCODING A HUMAN SKELETAL MUSCLE-SPECIFICUBIQUITIN-CONJUGATING ENZYME HOMOLOGOUS TO UBC7 OF C-ELEGANS, Cytogenetics and cell genetics, 74(1-2), 1996, pp. 146-148
Citations number
12
Categorie Soggetti
Cell Biology","Genetics & Heredity
Journal title
Cytogenetics and cell genetics → ACNP
ISSN journal
03010171
Volume
74
Issue
1-2
Year of publication
1996
Pages
146 - 148
Database
ISI
SICI code
0301-0171(1996)74:1-2<146:MOUEAH>2.0.ZU;2-0
Abstract
From a human fetal-brain cDNA library we isolated a novel ubiquitin-co njugating enzyme. The cDNA, designated UBE2G, contained an open readin g frame of 510 nucleotides encoding 170 amino acids. The predicted pep tide product showed 74% identity at the amino acid level with UBC7 of C. elegans, a high degree of homology with UBC7s of other species, and significant homologies with other subgroups of UBCs, Northern-blot an alysis revealed strong expression of 4.4-kb, 2.4-kb, and 1.6-kb transc ripts in skeletal muscle. Weak expression was observed in 15 other tis sues examined. Radiation-hybrid mapping localized this cDNA to chromos ome band 1q42.