CHROMOSOMAL ASSIGNMENT AND TISSUE EXPRESSION OF CBF-C NFY-C, THE 3RD SUBUNIT OF THE MAMMALIAN CCAAT-BINDING FACTOR/

The mammalian CCAAT-binding factor CBF (NFY) consists of three subunit s, CBF-A, CBF-B, and CBF-C. All three subunits are evolutionarily cons erved and are essential for DNA binding of CBF. In this study we repor t the identification of human and plant homologs of CBF-C. Northern an alysis revealed that, like the other two subunits, CBF-C was produced at equal levels in all rat tissues that were examined. We assigned the mouse CBF-C gene (designated Nfyc) to chromosome 4 with tight linkage to Lmyc. Our mouse linkage data suggest that the human NFYC homolog w ill map to 1p32. (C) 1996 Academic Press, Inc.