SELECTED LOCUS AND MULTIPLE PANEL MODELS FOR RADIATION HYBRID MAPPING

We develop two new types of models for whole-genome radiation hybrid m apping using the general multipoint framework. The first, selected loc us models, are appropriate for mapping markers in the region of a sele ctable locus that was used in creation of the hybrids. The models allo w for strong retention of the selectable locus, with retention rates d ecreasing with increasing distance from the selectable locus in both d irections. We illustrate the application of these models with 10 chrom osome 17 sequence-tagged site (STS) markers and the thymidine kinase ( TK) locus typed on a whole-genome hybrid panel in which TK was used in the selection process. The second set of models are appropriate when loci typed on two or more independent panels are to be used to build m aps. Maps can be built assuming interlocus distances are independent o r proportional between the panels, and the hypothesis of proportional distances can be tested. We illustrate the application of these models by using 27 chromosome 21 STS markers typed on two hybrid panels crea ted with radiation doses of similar to 10,000 and similar to 50,000 Ra ds.