INTERLEUKIN-3 (IL-3) AND GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR (GM-CSF) CAN INDUCE DIFFERENTIATION OF CHRONIC B-CELL LEUKEMIA EXPRESSING THE ALPHA-SUBUNIT OF IL-3 AND GM-CSF RECEPTOR

Citation
M. Shiiba et al., INTERLEUKIN-3 (IL-3) AND GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR (GM-CSF) CAN INDUCE DIFFERENTIATION OF CHRONIC B-CELL LEUKEMIA EXPRESSING THE ALPHA-SUBUNIT OF IL-3 AND GM-CSF RECEPTOR, International archives of allergy and immunology, 111, 1996, pp. 12-15
Citations number
8
Categorie Soggetti
Allergy,Immunology
ISSN journal
10182438
Volume
111
Year of publication
1996
Supplement
1
Pages
12 - 15
Database
ISI
SICI code
1018-2438(1996)111:<12:I(AGCF>2.0.ZU;2-A
Abstract
Interleukin-5 (IL-5), IL-3 and granulocyte-macrophage colony-stimulati ng factor (GM-CSF) exhibit similar functions on eosinophils, and these common functions are believed to be mediated by the shared beta chain of receptors. IL-5 shows activity on the murine chronic B cell leukem ia cell line BCL(1)-B20, inducing differentiation into IgM-secreting c ells, but IL-3 and GM-CSF do not have such activity. To elucidate whet her the lineage specificity of IL-5 is due to restricted expression of the IL-5 receptor a chain (IL-5R alpha), transfectants of BCL(1)-B20 were established that express IL-3 receptor alpha (BCL(1)-3R) or GM-CS F receptor alpha (BCL(1)-GMR). BCL(1)-3R and BCL(1)-GMR acquired respo nsiveness to IL-3 and GM-CSF, respectively, to an extent similar to IL -5 stimulation, resulting in IgM-secreting cells. Thus, the differenti ation of BCL(1)-B20 into IgM-secreting cells can be equally supported by either IL-3 or GM-CSF, suggesting that intracellular signaling thro ugh IL-5R can be replaced by signaling from IL-3R and GM-CSFR. These r esults support the notion that the lineage specificity of IL-5 is main ly due to the restricted expression of IL-5R alpha. Regulation of IL-5 R alpha, IL-3R alpha and GM-CSFR alpha expression in the developmental stage appears to be important for understanding the unique function o f these cytokines on a particular cell type.