CLONING AND SEQUENCING OF THE GROUP-6 ALLERGEN OF DERMATOPHAGOIDES-PTERONYSSINUS

Background The allergen Der p 6 from Dermatophagoides pteronysinus has been described by substrate affinity as mite chymotrypsin. Objective The aim of this paper was to describe a cDNA clone encoding the allerg en. Methods cDNA was cloned from a lambda library using oligonucleotid es published for Der p 6. Results The clone P6.1.1 had the N-terminal amino acid residues as reported for Der p 6, and at position 189 the S er was conserved in chymotrypsin for substrate specificity as well as the catalytic triad of His57, Asp102 and Ser195 for serine proteases. The estimated Mr was 24.9 K and it was 37% identical to the trypsin al lergen Der p3. Conclusion cDNA encoding Der p 6 has been described.