N. Songsasen et al., LAMBS PRODUCED FROM CRYOPRESERVED SHEEP EMBRYOS DERIVED BY IN-VITRO FERTILIZATION OF ASPIRATED OOCYTES, Canadian journal of animal science, 76(3), 1996, pp. 465-467
A total of 104 oocytes were aspirated from 1.1 mature ewes and 4 prepu
bertal lambs. After in vitro maturation, fertilization and culture, 11
1 of the 104 oocytes developed into blastocysts. These were cryopreser
ved in 1.5 M ethylene glycol. After being thawed, all were transferred
into four recipients, two of which became pregnant and delivered a no
rmal lamb.