PLATELET PLASMA-MEMBRANE IN SUBJECTS WITH PRIMARY NOCTURNAL ENURESIS - EFFECT OF DESMOPRESSIN

Plasma membrane fluidity of platelets (PLT) obtained from subjects wit h primary nocturnal enuresis (PNE) and healthy controls was investigat ed before and after addition of desmopressin (DDAVP). Membrane fluidit y was studied by measuring steady state fluorescence anisotropy of 1-( 4-trimethylammoniumphenyl)-6-phenyl-1,3,5 incorporated into PLT plasma membrane. Our results show an increase in membrane fluidity at the su rface level of PLT from subjects with PNE. Moreover, the addition of D DAVP induces a stable and significant decrease of membrane fluidity in both groups. These results suggest alterations of the lipid order in the exterior part of the PLT plasma membrane from patients with PNE.