ANALYSIS OF NATURALLY-OCCURRING ALPHA-ACETOTRIACYLGLYCERIDES BY GAS-CHROMATOGRAPHY CHEMICAL-IONIZATION MASS-SPECTROMETRY

The seed oils of three Maytenus species have been examined by high tem perature GLC using SOP-50 (methoxy-terminated) and OV-225-OH (OH-termi nated) glass capillary columns. The main compounds (71-76%) could be i dentified as alpha-mono-acetotriacylglycerols by a combination of high temperature GLC-MS in negative chemical ionization mode with a CH4/N2 O mixture as reactant gas and other spectroscopical methods (infrared, H-1- and C-13-nuclear magnetic resonance spectroscopy). The OV-225-OH phase demonstrated better separation properties than the SOP-50 phase for this substance class and up to eight species of alpha-monoacetotr iacyl-glycerols could be separated, The content of usual triacyclglyce rols was below 1%. The results of the analysis of the individual fatty acids as their methyl esters by GC and GC-MS are also presented.