SUPERCRITICAL-FLUID EXTRACTION OF FUMONISIN B-1 FROM GRAIN DUST

Fumonisin B-1 (FB1) was extracted from corn, corn dust, and Fusarium m oniliforme culture samples using supercritical carbon dioxide (SC-CO2) . The supercritical fluid extraction (SFE) conditions were optimized r egarding the use of different SC-CO2 modifiers, modifier volume, press ure, temperature, and static extraction time. The addition of the modi fier both in the extraction cell (prior to the static extraction step) and on-line with liquid carbon dioxide (during the dynamic SFE step) was found to significantly increase the recovery of FB1. Under the opt imized SFE conditions (15 mL of liquid CO2, 750 mu L of 5% acetic acid per gram of sample, 1200 psi, 20 min static extraction time), the rec overy of FB1 was found to be approximately 40 times greater than the r ecovery using conventional solvent extraction. SFE was faster and more reproducible (RSD = 3-5%) compared to the solvent extraction (RSD = 6 .5%). The calculated detection limit was approximately 150 ppb, on the basis of the HPLC analysis of FB1-fluorescamine derivatives with UV d etection.