EXPRESSION OF RSVLACZ AND PCMVLACZ RECOMB INANT-DNA IN PREIMPLANTATION RABBIT EMBRYOS

Authors
GOGOLEVSKII RA GOGOLEVSKAYA IK KUZNETSOVA IV KUZNETSOV AV
Citation
Ra. Gogolevskii et al., EXPRESSION OF RSVLACZ AND PCMVLACZ RECOMB INANT-DNA IN PREIMPLANTATION RABBIT EMBRYOS, Genetika, 32(8), 1996, pp. 1045-1050
Citations number
32
Categorie Soggetti
Genetics & Heredity
Journal title
Genetika → ACNP
ISSN journal
00166758
Volume
32
Issue
8
Year of publication
1996
Pages
1045 - 1050
Database
ISI
SICI code
0016-6758(1996)32:8<1045:EORAPR>2.0.ZU;2-L
Abstract
A linear fragment of the pRK3lacZ plasmid (RSVlacZ) and circular DNA o f pCMVlacZ were injected into rabbit zygotes. The embryos were culture d for 24-72 h and analyzed with chromogenic X-gal substrate for bacter ial beta-galactosidase. The expression of RSVlacZ DNA was revealed in 6-16-cell embryos, The expression of pCMVlacZ plasmid was low in 2-bla stomere embryos, and high in 4-16-cell embryos. In both cases, no beta -galactosidase activity was observed in one-cell embryos. The results obtained indirectly suggest selective and stepwise gene activation in the course of rabbit embryo development.