REGULATION OF TRYPTOPHAN BIOSYNTHESIS IN OBLIGATE METHYLOTROPHIC BACTERIUM METHYLOBACILLUS MUCOGENES

Authors
DOBROZHINETSKAYA EV MAKSIMOVA NP FOMICHEV YK
Citation
Ev. Dobrozhinetskaya et al., REGULATION OF TRYPTOPHAN BIOSYNTHESIS IN OBLIGATE METHYLOTROPHIC BACTERIUM METHYLOBACILLUS MUCOGENES, Genetika, 32(8), 1996, pp. 1056-1060
Citations number
24
Categorie Soggetti
Genetics & Heredity
Journal title
Genetika → ACNP
ISSN journal
00166758
Volume
32
Issue
8
Year of publication
1996
Pages
1056 - 1060
Database
ISI
SICI code
0016-6758(1996)32:8<1056:ROTBIO>2.0.ZU;2-F
Abstract
Twenty tryptophan-dependent mutants of obligate methylotrophic bacteri a Methylobacillus mucogenes M75 were obtained and characterized. Studi es of regulation of tryptophan synthesis in these bacterial cells show ed that genes trpE, trpD, and trpC undergo repression, and synthesis o f the trpBA gene product is constitutive. Anthranilate-synthase was fo und to be sensitive to feedback inhibition. It was ascertained that a 50% decrease in the activity of this enzyme occurs at a tryptophan con centration of 10(-4) M.