Ai. Emets et al., SOMATIC HYBRIDS OF HIGHER-PLANTS OBTAINED FROM AMIPROPHOSMETHYL-RESISTANT MUTANT NICOTINIANA-PLUMBAGINIFOLIA, Genetika, 32(8), 1996, pp. 1104-1111
Interspecific and intertribal somatic hybrids were obtained to study t
he composition and function of microtubules in hybrid plants. The amip
rophosmethyl-resistant mutant Nicotiana plumbaginifolia L. was used as
donor; canamycin-resistant mutants N. sylvestris L. and Atropa bellad
onna served as recipients. Cytogenetic analysis confirmed the hybrid n
ature of the clones selected. Immunoflourescent analysis showed that c
onstitutions of mitotic spindles in regenerating protoplast, isolated
from the hybrid NpAb-107 and the mutant N. plunbaginifolia, show no ch
ange after a 2-hour treatment with 5 mu M of amiprophosmethyl; in A. b
elladonna, the division spindle is completely destroyed under these co
nditions. Tubulin was isolated from the hybrid NpAb-107 and separated
by two-dimensional electrophoresis. The results showed that NpAb-107 h
as the beta-tubulin isoform specific for N. plumbaginifolia in additio
n to all isoforms of A. belladonna.