EFFECT OF SOME PARAMETERS ON ENANTIOMER SEPARATION OF EPHEDRINE, METHAMPHETAMINE AND SELEGILINE USING HPLC WITH BETA-CYCLODEXTRIN STATIONARY-PHASE

The influence of different parameters (mobile phase composition - pH, organic solvent, salt nature and concentration; flow rate, injected am ount and temperature) on enantiomeric separation of three pharmacologi cally important compounds (ephedrine, methamphetamine and selegiline) was studied using beta-cyclodextrin stationary phase. The evaluation o f effect of these parameters allowed to optimize condition for optical purity determination. The following experimental conditions were chos en for separation of racemic mixture: stationary phase - ChiraDex, 5 m u m; column - LiChroCart 250 x 4 mm I.D.; mobile phase - 500 mmol trie thylamine/l with H2SO4 in water, pH = 3.5, flow-rate - 0.8 mL/min; det ection - UV absorption at 206 nm; temperature - ambient. The separatio n of minor (1%) enantiomer in excess of major one can be improved usin g flow-rate 0.2 mL/min and thermostated column (20 degrees C for metha mphetamine and selegiline, 5 degrees C for ephedrine).