K. Lemr et al., EFFECT OF SOME PARAMETERS ON ENANTIOMER SEPARATION OF EPHEDRINE, METHAMPHETAMINE AND SELEGILINE USING HPLC WITH BETA-CYCLODEXTRIN STATIONARY-PHASE, Journal of liquid chromatography & related technologies, 19(19), 1996, pp. 3173-3191
Citations number
18
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
The influence of different parameters (mobile phase composition - pH,
organic solvent, salt nature and concentration; flow rate, injected am
ount and temperature) on enantiomeric separation of three pharmacologi
cally important compounds (ephedrine, methamphetamine and selegiline)
was studied using beta-cyclodextrin stationary phase. The evaluation o
f effect of these parameters allowed to optimize condition for optical
purity determination. The following experimental conditions were chos
en for separation of racemic mixture: stationary phase - ChiraDex, 5 m
u m; column - LiChroCart 250 x 4 mm I.D.; mobile phase - 500 mmol trie
thylamine/l with H2SO4 in water, pH = 3.5, flow-rate - 0.8 mL/min; det
ection - UV absorption at 206 nm; temperature - ambient. The separatio
n of minor (1%) enantiomer in excess of major one can be improved usin
g flow-rate 0.2 mL/min and thermostated column (20 degrees C for metha
mphetamine and selegiline, 5 degrees C for ephedrine).