Penicillium roqueforti (27%), P. corylophilum (20%) and Eurotium speci
es (15%) made up the important mycoflora associated with rye bread on
3425 identified fungi isolates. These fungi were dominant as spoilers
of packaged rye bread in almost every month of a 4 year investigation.
Penicillium decumbens (3%), Paecilomyces variotii (8%) and Aspergillu
s flavus (5%) were found more rarely, but were the major species found
over periods of a few months. Penicillium commune (5%), P. solitum (4
%), A. niger (4%) and Mucor species (2%) were a constant but small, pa
rt of the total mycoflora of rye bread. Identification of the fungi in
the production environment in a rye bread factory showed the locality
of potential contamination sources. Elimination of the contamination
sources by improved cleaning and disinfection procedures quickly resul
ted in a significant reduction in the frequency of mould growth in the
packaged rye bread.