KINETIC CHARACTERISTICS OF 1-EN-DEHYDROGENATION OF 6-ALPHA-METHYLHYDROCORTISONE BY CELLS OF ARTHROBACTER-GLOBIFORMIS-193

Biochemical characteristics of 6 alpha-methylhydrocortisone 1-en-dehyd rogenation by bacterial cells of Arthrobacter globiformis 193 have bee n studied. The reaction follows the kinetics of substrate inhibition; the inhibition reveals itself at the level of the respiratory chain. A mathematical model describing multiple substrate inhibition during th e process of 6 alpha-methylhydrocortisone 1-en-dehydrogenation with wh ole cells was proposed The solution of the constructed model agrees we ll with experimental data. There is little or no inhibitory effect at low substrate concentrations, and the reaction rate is determined by t he enzyme-substrate interaction rather than the respiratory chain acti vity. (C) 1996 Elsevier Science Inc.